Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and phosphorescence) lifetime imaging. The webinar will shortly reintroduce the principle of photoluminescence lifetime imaging. Based on the principle of frequency-domain luminescence lifetime imaging, the features of the new pco.flim camera will be discussed. Further, the simple experimental setup with a microscope in conjunction with an appropriate excitation light source will be shown. Based on experimental results with Förster resonance energy transfer (FRET) and endogenous fluorescence applications, the advantages and limitations of the new FLIM system will be presented.
Webinar: pco.flim pdf
Link to: Webinar at photonics.com