luminescence lifetime imaging camera

The pco.flim is the first Frequency Domain FLIM camera using a two tap CMOS sensor. Synchronized modulation of pixels and stimulated light enables you the analysis of luminescence decay times in the range of 100 ps – 100 µs.

With its 1008 x 1008 pixels resolution the pco.flim reads out 45 double frames/s at a max. You can use it for a modulation frequency range of 5 kHz – 40 MHz.

Using C-mount as standard the system is easy to connect to any microscope or lens. Further, the USB 3.0 interface lets you connect the camera to all kinds of computers. This saves you significant efforts and costs for operation and research.

The pco.flim laser is a homogeneously illuminating light source designed for use with the pco.flim. It features a digital modulation frequency range of 0 – 250 MHz. You can either order the pco.flim laser for widefield epifluorescence microscope illumination, or for Total-Internal-Reflection-Fluorescence (TIRF) microscopy, light sheet fluorescence microscopy (LSFM) and confocal spinning disk microscopy.

Choose between a wide range of different wavelengths and light output powers. The laser is ideally suited if your relevant luminescence lifetimes are in the range of a few nanoseconds. In case you are using a Nikon microscope, the Nikon double-safety-shutter option is also available.

contact data sheet whitepaper video inscoper integration


  • pco.flim front
  • pco.flim rear
  • pco.flim blue gray
  • pco.flim laser as light source for epifluorescence measurements 
  • selectable laser wavelengths and light output powers
  • 100 ps – 100 µs lifetimes measurable
  • 5 kHz – 40 MHz modulation frequencies
  • 500 kHz – 40 MHz external modulation signals
  • modulation signal shape sinusoidal / rectangular
  • 1008 x 1008 pixel resolution
  • frequency domain FLIM
  • USB 3.0 interface
  • 39 % quantum efficiency
  • 1000:1 dynamic range
  • 45 double frames/s (2 tap readout)
  • 1 ms to 2 s selectable exposure times
  • vibration-free water cooling
  • special software for measurement and analysis

contact data sheet

  • parameter unit setpoint pco.flim
    resolution (hor x ver) pixel 1008 x 1008
    pixel size (hor x ver) µm 5.6 x 5.6
    modulation frequency (out) 5 kHz - 40 MHz
    modulation frequency (in) 500 kHz - 40 MHz
    peak quantum efficiency % 39
    dynamic range A/D bit 14